CAS：4264-83-9 | The Application of 4-Nitrophenyl Phosphate (p-NPP) in IVD reagents

Product Name：4-Nitrophenyl Phosphate (p-NPP)
CAS：4264-83-9
Molecular Formula： C6H4NO6PNa2×6 H2O
Article No.： 10002602
Structural Formula：

Product Introduction:
4-Nitrophenyl Phosphate (p-NPP) belongs to functional fine chemical new materials.

4-Nitrophenyl Phosphate (p-NPP) is a classic and widely used synthetic phosphate ester substrate, which is mainly applied to the activity detection of various phosphatases (such as alkaline phosphatase ALP, acid phosphatase ACP, etc.). Its unique chemical structure endows it with excellent reaction specificity. Under the catalytic action of phosphatases, it can be efficiently hydrolyzed to generate yellow 4-nitrophenol (p-NP). This product has a strong and stable absorption peak at the wavelength of 405 nm, which can be accurately quantified by spectrophotometry, thus indirectly reflecting the phosphatase activity level in the sample.

p-NPP, known as the "golden substrate" for phosphatase detection, with its core advantages of high specificity and high reaction efficiency, is widely used in various fields such as in vitro diagnostic reagent preparation and life science research, and is compatible with different detection technology platforms.

1，In Vitro Diagnostic (IVD) Reagent Field

This is the core application scenario of p-NPP, which is mainly used in the preparation of diagnostic reagents for alkaline phosphatase (ALP), acid phosphatase (ACP) and other related enzymes. Specific applications include:

Reagents matched with fully/ semi-automatic biochemical analyzers

These reagents are used for detecting ALP/ACP activity in serum and plasma samples, providing accurate data for the clinical screening and diagnosis of hepatobiliary diseases, skeletal diseases, prostatic diseases, etc.

POCT rapid diagnostic reagents

Examples include dry chemical test strips and portable detection kits, which are suitable for point-of-care testing scenarios such as primary healthcare, emergency bedside testing and health check-ups.

Chromogenic substrate for enzyme-linked immunosorbent assay (ELISA)

As a matched chromogenic substrate for ALP-labeled antibodies, it is widely applied in the field of immunodiagnosis, including infectious disease detection, tumor marker detection and endocrine detection.

2，Life Sciences and Scientific Research Field: Used in biochemistry and molecular biology research for experiments such as phosphatase activity determination, enzyme kinetics analysis, and inhibitor screening; at the same time, it can serve as a substrate for identifying phosphatase-positive strains in microbial detection, facilitating microbiological research and clinical microbial diagnosis.

3， Other industrial and testing fields: It can be used for the rapid detection of phosphatase activity in the food industry (such as the detection of alkaline phosphatase residues in dairy products to ensure food hygiene and safety); it can also serve as the core sensitive material of biosensors to construct devices for the rapid detection of phosphatase activity.

Advantages：

1, Stable Performance & Long Shelf Life:

Thanks to optimized molecular structure design and strict production environment control, the product boasts excellent stability. Under standard storage conditions, it has a shelf life of up to 24 months, effectively reducing customers’ inventory losses and production costs.

2, Large-scale Production & Stable Supply:

Equipped with modern production bases and automated production lines. We can quickly respond to large-batch order demands and ensure the continuity of customers’ production operations.

3, Quality Assurance: Full-process Control from R&D to Delivery:

- Composed of a team of senior experts in the IVD raw materials field, we are committed to continuous in-depth technological innovation, ensuring the technological leadership, stability and cost-effectiveness of our products.

- Our production workshops comply with GMP standards.

- Equipped with advanced testing equipment including High-Performance Liquid Chromatography (HPLC), ultraviolet spectrophotometers and stability test chambers, every batch of products undergoes comprehensive testing of multiple indicators such as purity and stability, and is only allowed to leave the factory upon passing all tests.

About Samples and Logistics
You can test before purchasing; free samples (EXW only) of less than 5g are available. For mass orders, FOB or CFR delivery is available. Please check the goods upon receipt; contact us immediately if there is any damage. For other express or logistics needs, please contact us.