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EPS-G7: The Core Substrate for α-Amylase Assay Reagents

3-(2'-Spiroadamantane)-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane

EPS-G7: The Core Substrate for α-Amylase Assay Reagents

Apr 30, 2026

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Qianyin New Material has grown into a national high-tech enterprise integrating R&D, production, sales, and services through years of innovation and development. Its business scope covers life sciences, in vitro diagnostic raw materials, new materials, e-commerce for scientific research procurement, testing, and green energy.

Sarah M.

The detection result of α-amylase is of great reference value for the diagnosis of various diseases, such

as acute pancreatitis, acute abdomen, chronic pancreatitis, etc. EPS-G7 is precisely the key substrate for

this detection. According to the recommendation of the International Federation of Clinical Chemistry and

Laboratory Medicine (IFCC), ethylidene-4-NP-G7 should be used in diagnostic tests for the determination of

α-amylase and pancreatic α-amylase.

EPS-G7 is 4,6-Ethylidene-4-nitrophenyl-1,4-α-D-maltoheptaoside, CAS 96597-16-9, molecular formula is C₅₀H₇₇NO₃₈, and molecular weight is 1300.1. This substrate is specifically designed for α-amylase activity measurement. The introduction of an ethylidene protecting group significantly enhances its specificity for α-amylase.

EPS-G7 is primarily used in α-amylase assay kits for clinical biochemistry. The core reaction principle is as follows: α-amylase in the sample hydrolyzes the α-1,4 glycosidic bonds of the substrate. Through a series of coupled enzymatic reactions (typically involving α-glucosidase), 4-nitrophenol (PNP) is ultimately released. By monitoring the rate of PNP production at 405 nm (kinetic method), the α-amylase activity in the sample can be accurately calculated.

By choosing our EPS-G7 substrate, you gain the following core advantages:

Extremely Low Blank Absorbance & Stable Blank Rate
Excellent Linear Range
Superior Stability
Strong Interference Resistance
Strict Batch-to-Batch Consistency

Choosing a high-purity, low-blank, kinetically superior EPS-G7 substrate is the foundation for developing high-quality α-amylase detection reagents. As a professional IVD raw material supplier, we offer batch-stable EPS-G7 products with complete Certificates of Analysis (COA) and support flexible, customized packaging specifications to help reagent manufacturers shorten development cycles and enhance product competitiveness.

Welcome to request technical documentation and samples. We are dedicated to providing you with professional support and service.

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