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BCIP/NBT Purple Enhanced One Component Membrane Substrate produces an enhanced dark purple signal and is suitable for applications including Western blot and microarray analysis.
Item no: BCPET
Package size: 1L, 5L, 10L, custom
pH value: 9.8+/-0.3
Shelf life: 3 years
Storage: 2-8C
Application:
Western blot, Northern blot, Microarray, ELISpot etc.
Brand :
QYMItem No :
BCPETOrder(MOQ) :
On RequestPayment :
T/TProduct Origin :
ChinaColor :
Transparent, and large volumes may appear light yellow or light blue.Shipping Port :
Hefei or ChinaLead Time :
On RequestWeight :
On RequestQYM/SURFTECH series of BCIP/NBT membrane/precipitation substrates feature low background, high stability, sensitivity, and minimal batch-to-batch variation. The series provides blue and purple signal systems, along with BCIP/NBT substrates dedicated for immunohistochemistry (IHC). BCIP/NBT Purple Enhanced One Component Membrane Substrate produces an enhanced dark purple signal and is suitable for applications including Western blot and microarray analysis.
Item no: BCPET
Package size: 1L, 5L, 10L, custom
pH value: 9.8+/-0.3
Shelf life: 3 years
Storage: 2-8C
Application:
Western blot, Northern blot, Microarray, ELISpot etc.
Instruction:
QYM/SURFTECH BCIP/NBT Purple Enhanced One Component Membrane Substrate is ready-to-use and should be equilibrated to the reaction temperature before application.
Chromogenic Reaction Procedure (Western Blot):
1. Wash: After alkaline phosphatase (AP) conjugate incubation, wash the membrane 3 times with TBST washing buffer.
2. Chromogenic Reaction: Add 1 mL substrate and incubate at 20-37 C until bands appear dark purple.
3. Stop: Wash 2-3 times with 1.5 mL deionized water to terminate the reaction before background color develops.
4. Detection: Analyze bands by visual inspection or by imaging to quantify grayscale values.
For research use only or for further use in the production of detection kits. Not for human or animal use!
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BCIP/NBT Purple Enhanced One Component Membrane Substrate produces an enhanced dark purple signal and is suitable for applications including Western blot and microarray analysis. Item no: BCPET Package size: 1L, 5L, 10L, custom pH value: 9.8+/-0.3 Shelf life: 3 years Storage: 2-8C Application: Western blot, Northern blot, Microarray, ELISpot etc.
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